Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells, Raji cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
80019-1-RR targets IkB Alpha in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag0345 Product name: Recombinant human NFKBIA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 19-237 aa of BC002601 Sequence: GLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLL Predict reactive species |
| Full Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa and 35 kDa |
| GenBank Accession Number | BC002601 |
| Gene Symbol | IkB Alpha |
| Gene ID (NCBI) | 4792 |
| RRID | AB_2882942 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P25963 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. IkB alpha can be observed as a band at 36 kDa in human cell lysates, and 35 kDa in mouse and rat cell ysates.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IkB Alpha antibody 80019-1-RR | Download protocol |
| WB protocol for IkB Alpha antibody 80019-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IkB Alpha Recombinant monoclonal antibody (Cat# 80019-1-RR) has 8 citations. These appear in Carbohydrate Polymers, Journal of Neuroinflammation, International Journal of Pharmaceutics X, Journal of Ethnopharmacology, European Journal of Pharmacology, International Immunopharmacology, Journal of Pharmaceutical and Biomedical Analysis, and American Journal of Translational Research. Research fields span wound healing and immunomodulation, Alzheimer's disease and neuroinflammation, pain relief, intestinal immunity, inflammatory bowel disease, anti-inflammatory mechanisms, and hepatic fibrosis, with most studies utilizing Western blot in mouse models.
| Species | Application | Title |
|---|---|---|
Carbohydr Polym pH-responsive and self-adaptive injectable sodium alginate/carboxymethyl chitosan hydrogel accelerates infected wound healing by bacteriostasis and immunomodulation | ||
J Neuroinflammation IL-27, a metabolic regulator secreted by astrocytes in response to GLP-1RA OHP2, modulates microglial reprogramming in Alzheimer's disease by regulating cGAS lactylation. | ||
Int J Pharm X A synergistic nanozyme platform breaking the neuroinflammatory-oxidative stress cycle for extended pain relief. | ||
J Ethnopharmacol Mechanistic Insights into the Effects of Norisoboldine on Intestinal Immunity and Neuroinflammation in a Mouse Model of Slow Transit Constipation with Depression | ||
Eur J Pharmacol Capsaicin attenuates DSS-induced IBD by suppressing macrophage M1 polarization via inhibition of the TRAF1/NF-κB pathway. | ||
Int Immunopharmacol Preventive effects of matrine on LPS-induced inflammation in RAW 264.7 cells and intestinal damage in mice through the TLR4/NF-κB/MAPK pathway |
