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Non-treated SH-SY5Y cells, phosphatase inhibitor treated SH-SY5Y cells and λ phosphatase treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 80101-7-RR (Phospho-TAU (Thr231) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Non-treated SH-SY5Y cells, phosphatase inhibitor treated SH-SY5Y cells and λ phosphatase treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 80101-7-RR (Phospho-TAU (Thr231) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.
IHC staining of human brain（AD） using 80101-7-RR
Immunohistochemical analysis of paraffin-embedded human brain(AD) slide using 80101-7-RR (Phospho-TAU (Thr231) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Immunohistochemical analysis of paraffin-embedded human brain(AD) slide using 80101-7-RR (Phospho-TAU (Thr231) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 80101-7-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Phospho-TAU (Thr231) antibody (80101-7-RR, Clone: 240632B1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded 3xTg AD mouse brain tissue using Phospho-TAU (Thr231) antibody (80101-7-RR, Clone: 240632B1 ) at dilution of 1:300 and Multi-rAb CoraLite Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H + L) (RGAR002). Image credit: Ross McNally, PhD, Northwestern University.
FC experiment of SH-SY5Y using 80101-7-RR
1X10^6 SH-SY5Y cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-TAU (Thr231) Recombinant antibody (80101-7-RR, Clone:240632B1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
1X10^6 SH-SY5Y cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-TAU (Thr231) Recombinant antibody (80101-7-RR, Clone:240632B1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.
FC experiment of SH-SY5Y using 80101-7-RR
1x10^6 SH-SY5Y cells were intracellularly stained with 0.06 ug Phospho-TAU (Thr231) Recombinant antibody (80101-7-RR, Clone:240632B1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-2-RR, Clone: 240953A11) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
1x10^6 SH-SY5Y cells were intracellularly stained with 0.06 ug Phospho-TAU (Thr231) Recombinant antibody (80101-7-RR, Clone:240632B1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-2-RR, Clone: 240953A11) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.
human brain（AD） Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse brain tissue
Positive IF-Frozen detected in
mouse brain tissue
Positive FC (Intra) detected in
SH-SY5Y cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:4000
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)-P
IF-P : 1:250-1:1000
Immunofluorescence (IF)-FROZEN
IF-FROZEN : 1:150-1:600
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
80101-7-RR targets Phospho-TAU (Thr231) in WB, IHC, IF-P, FC (Intra), ELISA, IF-Frozen applications and shows reactivity with human, mouse samples.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. The Tau protein has six isoforms produced from a single gene through alternative RNA splicing. Isoforms differ in number of inserts at the N-terminal half and the number of repeats at the C-terminal half (3 repeat-3R; 4 repeat-4R). Tau is hyperphosphorylated during aging and in age-related neurodegenerative diseases such as Alzheimer's disease (AD) and fronto-temporal dementia. Hyperphosphorylation of Tau leads to the formation of neurofibrillary tangles (NFT) in the neurons and glia cells, which is one of the hallmarks of AD.
Protocols
Product Specific Protocols
IF protocol for Phospho-TAU (Thr231) antibody 80101-7-RR
This validation data has been generated in-house and reviewed by Proteintech scientists.
Find out more on our antibody validation policy
WB Figures
WB analysis using 80101-7-RR
Non-treated SH-SY5Y cells, phosphatase inhibitor treated SH-SY5Y cells and λ phosphatase treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 80101-7-RR (Phospho-TAU (Thr231) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.
IHC Figures
IHC staining of human brain（AD） using 80101-7-RR
Immunohistochemical analysis of paraffin-embedded human brain(AD) slide using 80101-7-RR (Phospho-TAU (Thr231) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P Figures
IF Staining of mouse brain using 80101-7-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Phospho-TAU (Thr231) antibody (80101-7-RR, Clone: 240632B1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 80101-7-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded λ phosphatase treated mouse brain tissue using Phospho-TAU (Thr231) antibody (80101-7-RR, Clone: 240632B1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-FROZEN Figures
IF Staining of mouse brain using 80101-7-RR
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded 3xTg AD mouse brain tissue using Phospho-TAU (Thr231) antibody (80101-7-RR, Clone: 240632B1 ) at dilution of 1:300 and Multi-rAb CoraLite Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H + L) (RGAR002). Image credit: Ross McNally, PhD, Northwestern University.
FC (INTRA) Figures
FC experiment of SH-SY5Y using 80101-7-RR
1X10^6 SH-SY5Y cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-TAU (Thr231) Recombinant antibody (80101-7-RR, Clone:240632B1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.
FC experiment of SH-SY5Y using 80101-7-RR
1x10^6 SH-SY5Y cells were intracellularly stained with 0.06 ug Phospho-TAU (Thr231) Recombinant antibody (80101-7-RR, Clone:240632B1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-2-RR, Clone: 240953A11) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Phospho-TAU (Thr231) Recombinant antibody
Catalog Number
80101-7-RR
Citations
1
Dilutions
WB : 1:1000-1:4000 IHC : 1:500-1:2000 IF-P : 1:250-1:1000 IF-FROZEN : 1:150-1:600 FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF-P, FC (Intra), ELISA, IF-Frozen
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
