Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, U-251 cells, U2OS cells, NIH/3T3 cells, HSC-T6 cells, 4T1 cells, ROS1728 cells |
| Positive IHC-Autostainer detected in | human melanoma tissue, human ovary cancer tissue, human liver tissue, human pancreas tissue, human stomach cancer tissue, human tonsillitis tissue, human appendicitis tissue, human kidney tissue |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue, human tonsillitis tissue, human ovary cancer tissue, human pancreas tissue, human placenta tissue, human stomach cancer tissue, human colon tissue, human renal cell carcinoma tissue, human kidney tissue, human appendicitis tissue, human colon cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-Fro detected in | mouse breast cancer |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC)-AUTOSTAINER | IHC-AUTOSTAINER : 1:250-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:1250-1:5000 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
80232-1-RR targets Vimentin in WB, IHC, IHC-Autostainer, IF/ICC, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag0489 Product name: Recombinant human Vimentin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-466 aa of BC000163 Sequence: MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE Predict reactive species |
| Full Name | vimentin |
| Calculated Molecular Weight | 466 aa, 54 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC000163 |
| Gene Symbol | VIM |
| Gene ID (NCBI) | 7431 |
| ENSEMBL Gene ID | ENSG00000026025 |
| RRID | AB_2918879 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08670 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Vimentin antibody 80232-1-RR | Download protocol |
| IF protocol for Vimentin antibody 80232-1-RR | Download protocol |
| IHC protocol for Vimentin antibody 80232-1-RR | Download protocol |
| WB protocol for Vimentin antibody 80232-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Vimentin Recombinant monoclonal antibody (6K21), catalog number 80232-1-RR, has 24 cited publications. Citations appear in journals including J Exp Clin Cancer Res, Pharmacol Res, Mol Ther, Mater Today Bio, Front Immunol, Mol Cancer Res, Sci Rep, BMC Cancer, J Neurooncol, and bioRxiv. Research fields span multiple cancer types (prostate, gastric, breast, endometrial, ovarian, bladder, colorectal, liver, glioma), pulmonary fibrosis, rheumatoid arthritis, cardiac repair, and immune microenvironment remodeling.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res A novel polypeptide encoded by circSPIRE1 promotes prostate cancer proliferation and migration by restraining the ubiquitin-dependent degradation of LRP5 | ||
Pharmacol Res Sinomenine regulates the AKT/FOXO3/GLUL pathway to inhibit pulmonary fibroblast-to-myofibroblast transition via α7nAChR against rheumatoid arthritis associated interstitial lung disease. | ||
Mol Ther Therapeutic delivery of albumin-binding siRNA targeting IRS2 to diverse cell types reduces mammary tumor growth. | ||
Mater Today Bio Self-assembled extracellular matrix-lipid nanoparticle composite for site-specific siRNA delivery to improve cardiac repair post-myocardial infarction. | ||
J Orthop Translat An iguratimod prodrug reduces RA-FLS invasiveness by disrupting STAT1-C3-TNFα-mediated crosstalk between fibroblast-like synoviocytes and macrophages. | ||
Int J Biol Macromol circGAPVD1 inhibits the progression of gastric cancer through miR-4424/STK4 axis and encoding GAPVD1-137aa protein |
Reviews
What customers say
The single review for this antibody (rated 4/5) was for immunofluorescence on mouse brain slices at a 1:1250 dilution. The reviewer reported good staining of vimentin-positive ependymal cell processes, though noted that staining in the cell bodies appeared somewhat weaker than in the processes. Overall, the antibody performed well for its intended application with only a minor limitation in signal intensity across different cellular compartments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sama (Verified Customer) (03-26-2026) | Nice staining of vimentin+ ependymal cell processes - staining in the cell bodies, however, appears weaker :(
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