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Phospho-YAP1 (Ser397) Recombinant monoclonal antibody (80694-3-RR)

Phospho-YAP1 (Ser397) Recombinant monoclonal antibody

Phospho-YAP1 (Ser397) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 80694-3-RR

Clone No. 250306F11

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

p YAP1 (Ser397), Phospho YAP1 (Ser397), Phospho-YAP, Phospho-YAP (Ser397), Phospho-YAP(Ser397)

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

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Filter: AllWBFC (Intra)

WB analysis of NIH/3T3 using 80694-3-RR
NIH/3T3 cell lysates were subjected to SDS PAGE followed by western blot with 80694-3-RR (Phospho-YAP1 (Ser397) antibody) blocked with BSA only, Phospho-YAP1 (Ser397) peptide or non-Phospho peptide at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 80694-3-RR
Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80694-3-RR (Phospho-YAP1 (Ser397) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of NIH/3T3 using 80694-3-RR
1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-YAP1 (Ser397) Recombinant monoclonal antibody (80694-3-RR, Clone:250306F11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-YAP1 (Ser397) Recombinant monoclonal antibody (80694-3-RR, Clone:250306F11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

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Overview

Tested Applications
Product Information
Protocols
Reviews (1)

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MSDS SDS

Tested Applications

Positive WB detected in	NIH/3T3 cells
Positive FC (Intra) detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80694-3-RR targets Phospho-YAP1 (Ser397) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	Yes-associated protein 1, 65kDa
Calculated Molecular Weight	504 aa, 54 kDa
Observed Molecular Weight	75 kDa
GenBank Accession Number	BC038235
Gene Symbol	YAP1
Gene ID (NCBI)	10413
RRID	AB_3743352
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P46937
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999)

Protocols

Product Specific Protocols
FC protocol for Phospho-YAP1 (Ser397) antibody 80694-3-RR	Download protocol
WB protocol for Phospho-YAP1 (Ser397) antibody 80694-3-RR	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Reviews

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What customers say

The product received a single 5-star review for Western Blot at a 1:5000 dilution. The reviewer reported a positive experience with no additional comments or images provided. Overall, the feedback indicates satisfactory performance for the tested application.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Li (Verified Customer) (10-08-2026)