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  • Western Blot (WB) analysis of NIH/3T3 cells using Phospho-YAP1 (Ser397) Recombinant antibody (80694-3-RR)

    WB analysis of NIH/3T3 using 80694-3-RR

    NIH/3T3 cell lysates were subjected to SDS PAGE followed by western blot with 80694-3-RR (Phospho-YAP1 (Ser397) antibody) blocked with BSA only, Phospho-YAP1 (Ser397) peptide or non-Phospho peptide at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Phospho-YAP1 (Ser397) Recombinant antibody

Phospho-YAP1 (Ser397) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 80694-3-RR
Clone No.250306F11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

p YAP1 (Ser397), Phospho YAP1 (Ser397), Phospho-YAP, Phospho-YAP (Ser397), Phospho-YAP(Ser397)

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80694-3-RR targets Phospho-YAP1 (Ser397) in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name Yes-associated protein 1, 65kDa
Calculated Molecular Weight 504 aa, 54 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC038235
Gene Symbol YAP1
Gene ID (NCBI) 10413
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP46937
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999)

Protocols

Product Specific Protocols
WB protocol for Phospho-YAP1 (Ser397) antibody 80694-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols