Tested Applications
| Positive WB detected in | HEK-293 cells, human urine exosomes, HeLa cells, K-562 cells, NIH/3T3 cells, C6 cells, the whole yeast, HEK-293 exosomes |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, human lung squamous cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
80735-1-RR targets HSP70 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, yeast samples.
| Tested Reactivity | human, mouse, rat, yeast |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag1446 Product name: Recombinant human HSP70 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 291-641 aa of BC009322 Sequence: IDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD Predict reactive species |
| Full Name | heat shock 70kDa protein 1A |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC009322 |
| Gene Symbol | HSP70 |
| Gene ID (NCBI) | 3303 |
| RRID | AB_2918908 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P0DMV8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HSP70 antibody 80735-1-RR | Download protocol |
| IHC protocol for HSP70 antibody 80735-1-RR | Download protocol |
| WB protocol for HSP70 antibody 80735-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HSP70 Recombinant monoclonal antibody (4E10), catalog number 80735-1-RR, has 5 total citations. These appear in Mater Today Bio, J Orthop Translat, J Med Chem, Cell Signal, and Leg Med (Tokyo). Research fields span heart failure therapy via plant exosomes, cuproptosis in osteoarthritis, antitumor drug discovery through PPIA interaction, cisplatin resistance in non-small cell lung cancer, and forensic assessment of fatal heatstroke in a rat model.
| Species | Application | Title |
|---|---|---|
Mater Today Bio From targeted delivery to inflammation suppression: engineering plant exosomes for heart failure therapy post-infarction. | ||
J Orthop Translat Cuproptosis in osteoarthritis: Exploring chondrocyte cuproptosis and therapeutic avenues | ||
J Med Chem Discovery of Novel Oxazolo[4,3-f]purine Derivatives as Antitumor Agents through PPIA Interaction | ||
Cell Signal LAPTM5 confers cisplatin resistance in NSCLC by suppressing LAMP1 ubiquitination to stabilize lysosomal membranes and sustain autophagic flux | ||
Leg Med (Tokyo) A preliminary multimodal forensic assessment of footpad skin in a rat model of fatal heatstroke. |
Reviews
What customers say
The single review for this product (lot 23001224, used for Western Blot) rated it 5 stars. The reviewer noted that the item arrived promptly and was well packaged. Overall, the feedback is positive, highlighting reliable shipping and good packaging, though no detailed comments on performance or results were provided.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tom (Verified Customer) (10-23-2025) | Item arrived promptly and well packaged
|
