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Phospho-BCL2 (Ser70) Recombinant monoclonal antibody (80771-2-RR)

Phospho-BCL2 (Ser70) Recombinant monoclonal antibody

Phospho-BCL2 (Ser70) Uni-rAb^® Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 80771-2-RR

Clone No.230061A8

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

BCL-2, BCL2, 230061A8

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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Filter: AllWBIHCFC (Intra)

WB analysis using 80771-2-RR
Non-treated Jurkat cells, paclitaxel treated and Calyculin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 80771-2-RR (Phospho-BCL2 (Ser70) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 80771-2-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80771-2-RR (Phospho-BCL2 (Ser70) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of Jurkat using 80771-2-RR
1x10^6 Jurkat cells untreated (dashed lines) or treated with Staurosporine which intracellularly stained with 0.06 ug Phospho-BCL2 (Ser70) Recombinant antibody (80771-2-RR, Clone:230061A8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 Jurkat cells untreated (dashed lines) or treated with Staurosporine which intracellularly stained with 0.06 ug Phospho-BCL2 (Ser70) Recombinant antibody (80771-2-RR, Clone:230061A8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, paclitaxel treated Jurkat cells, Calyculin A treated Jurkat cells
Positive IHC detected in	human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	Staurosporine treated Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80771-2-RR targets Phospho-BCL2 (Ser70) in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	B-cell CLL/lymphoma 2
Calculated Molecular Weight	26 kDa
Observed Molecular Weight	26 kDa
GenBank Accession Number	BC027258
Gene Symbol	BCL2
Gene ID (NCBI)	596
RRID	AB_3670487
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P10415
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL2 belongs to the Bcl-2 family. The anti-apoptotic protein BCL2 can bind to the pro-apoptotic protein BAX or BAK1 to form heterodimers that modulate apoptosis. BCL2 phosphorylation at Ser70 enhances binding to BAX and BAD and is essential for its anti-apoptotic activity. Conversely, BCL2 also regulates autophagy via interaction with BECN1/Beclin 1; moreover, BCL2 phosphorylation including at Ser70 leads to its dissociation from BECN1 and induction of autophagy. These findings suggest that BCL2 regulates the balance between apoptosis and autophagy. (PMID: 29433359)

Protocols

Product Specific Protocols
IHC protocol for Phospho-BCL2 (Ser70) antibody 80771-2-RR	Download protocol
WB protocol for Phospho-BCL2 (Ser70) antibody 80771-2-RR	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	Cell Death Dis NOD2 reduces the chemoresistance of melanoma by inhibiting the TYMS/PLK1 signaling axis Authors - Fang Yun View Article
human	WB	Genes Dis Identifying C1orf122 as a potential HCC exacerbated biomarker dependently of SRPK1 regulates PI3K/AKT/GSK3β signaling pathway. Authors - Jing Cai View Article