Tested Applications
| Positive WB detected in | LNCaP cells, HeLa cells, NCCIT cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
80790-1-RR targets METTL14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag14325 Product name: Recombinant human METTL14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 107-456 aa of BC007449 Sequence: LKGTQSLNPHNDYCQHFVDTGHRPQNFIRDVGLADRFEEYPKLRELIRLKDELIAKSNTPPMYLQADIEAFDIRELTPKFDVILLEPPLEEYYRETGITANEKCWTWDDIMKLEIDEIAAPRSFIFLWCGSGEGLDLGRVCLRKWGYRRCEDICWIKTNKNNPGKTKTLDPKAVFQRTKEHCLMGIKGTVKRSTDGDFIHANVDIDLIITEEPEIGNIEKPVEIFHIIEHFCLGRRRLHLFGRDSTIRPGWLTVGPTLTNSNYNAETYASYFSAPNSYLTGCTEEIERLRPKSPPPKSKSDRGGGAPRGGGRGGTSAGRGRERNRSNFRGERGGFRGGRGGAHRGGFPPR Predict reactive species |
| Full Name | methyltransferase like 14 |
| Calculated Molecular Weight | 456 aa, 52 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC007449 |
| Gene Symbol | METTL14 |
| Gene ID (NCBI) | 57721 |
| RRID | AB_2918912 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9HCE5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
METTL14, is also named as Methyltransferase-like protein 14 or KIAA1627, is a 456 amino acid protein, which belongs to the MT-A70-like family and localized in the nucleus. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues of some mRNAs and regulates the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naïve pluripotency-promoting transcripts results in transcript destabilization.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for METTL14 antibody 80790-1-RR | Download protocol |
| WB protocol for METTL14 antibody 80790-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
80790-1-RR (METTL3 antibody) has 11 total citations published in journals including Journal of Advanced Research, Journal of Clinical Investigation, International Journal of Biological Macromolecules, Free Radical Biology and Medicine, Frontiers in Immunology, Ecotoxicology and Environmental Safety, Cell Signaling, ACS Infectious Diseases, BBA Molecular and Cell Biology of Lipids, Frontiers in Genetics, and Cancer Reports. Research fields span pulmonary fibrosis, Parkinson's disease, rheumatoid arthritis, osteogenesis, renal injury, pyroptosis, infection, hepatic steatosis, aortic aneurysm, and hepatocellular carcinoma.
| Species | Application | Title |
|---|---|---|
J Adv Res Targeting myofibroblast copper vulnerability reverses pulmonary fibrosis via METTL3-directed STAT6 m6A driving cuproptosis. | ||
J Clin Invest m6A deficiency induces dopaminergic neurodegeneration and progressive parkinsonism through a pathogenic loop with mitochondria | ||
Int J Biol Macromol METTL3 increases ferroptosis resistance to facilitate the tumor-like features of rheumatoid arthritis synovial fibroblasts through enhancing SLC7A11 mRNA stability in an m6A-IGF2BP2-dependent manner | ||
Free Radic Biol Med Spermidine promotes osteogenic differentiation of BMSCs via the m(6)A methylation-autophagy axis under inflammatory stress. | ||
Front Immunol m6A epigenetic signature in necrotizing enterocolitis: insights from murine to neonatal studies. | ||
Ecotoxicol Environ Saf Changes of N(6)-methyladenosine modification and unfolded protein response in renal cell injury induced by cadmium. |
Reviews
What customers say
The single available review is a 5-star rating from a researcher who used this antibody for Western Blot on human lung cancer cell lines (Ludlu-1, H1299, A459). They reported it worked well with an overnight incubation at 4°C at a 1:1000 dilution and recommended the product.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nikolett (Verified Customer) (09-05-2025) | I recommend this antibody. Works well on human lung cancer cell lines after overnight incubation at 4°C.
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