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  • Western Blot (WB) analysis of various lysates using Phospho-c-MYC (Ser62) Recombinant monoclonal antib (80844-4-RR)

    WB analysis using 80844-4-RR

    Non-treated HEK-293T cells and MG132 treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 80844-4-RR (Phospho-c-MYC (Ser62) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • Western Blot (WB) analysis of HeLa cells using Phospho-c-MYC (Ser62) Recombinant monoclonal antib (80844-4-RR)

    WB analysis of HeLa using 80844-4-RR

    Non-treated HeLa cells and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 80844-4-RR (Phospho-c-MYC (Ser62) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Phospho-c-MYC (Ser62) Recombinant monoclonal antibody

Phospho-c-MYC (Ser62) Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 80844-4-RR
Clone No.250304G12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

c-Myc (phospho S62), c-MYC (Ser62), p-c-MYC (S62), p-c-MYC (Ser62), p-c-MYC Ser62

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inMG132 treated HEK-293T cells, MG132 treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80844-4-RR targets Phospho-c-MYC (Ser62) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

Predict reactive species
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated Molecular Weight 49 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC000141
Gene Symbol MYC
Gene ID (NCBI) 4609
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP01106
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYC is a transcription factor that globally enhances expression of transcribing genes, including those vital for cell cycle, growth, proliferation, and survival in normal and cancer cells. MYC has multiple isomers, and this antibody recognizes the phosphorylation of the 439 amino acid isomer (P01106-1, UniProt) at the 62 serine site. Posttranslational modifications that regulate MYC stability include phosphorylations at Ser62 and Thr58. Phosphorylation at Ser62, primarily by ERK, stabilizes MYC. While kinases other than ERK phosphorylate Ser62, GSK3β has been the only kinase known to phosphorylate MYC at Thr58.(PMID: 32482868)

Protocols

Product Specific Protocols
WB protocol for Phospho-c-MYC (Ser62) antibody 80844-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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