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c-MYC Recombinant monoclonal antibody, PBS Only (Detector) (80845-4-PBS)

c-MYC Recombinant monoclonal antibody, PBS Only (Detector)

Name: c-MYC Recombinant monoclonal antibody, PBS Only (Detector), 100ug
Brand: Proteintech
SKU: 80845-4-PBS-100UG

c-MYC Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Cat No. 80845-4-PBS

Clone No. 261876G5

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA

Synonyms

MYC, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39, MRTL

Formulation: PBS Only

PBS Only

Conjugate: Unconjugated

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Filter: AllSandwich ELISA sample testSandwich ELISA

Sandwich ELISA sample test of MP03858-1
The mean c-MYC concentration was determined to be 1.02 ng/mL in Raji cell extract based on a 1.20 mg/mL extract load, 1.29 ng/mL in HL-60 cell extract based on a 1.20 mg/mL extract load and 0.52 ng/mL in HT-1080 cell extract based on a 1.20 mg/mL extract load.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sandwich ELISA sample test of MP03858-1
Serum of eight individual healthy human donors was measured. The c-MYC concentration of detected samples was determined to be 0.46 ng/mL with a range of 0.03-2.72 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sandwich ELISA standard curve of MP03858-1
Sandwich ELISA standard curve of MP03858-1, Human c-MYC Recombinant Matched Antibody Pair - PBS only. 80845-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1263. 80845-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
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Product Information

80845-4-PBS targets c-MYC as part of a matched antibody pair:

MP03858-1: 80845-2-PBS capture and 80845-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag1263 Product name: Recombinant human MYC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 16-454 aa of BC000141 Sequence: MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA Predict reactive species
Full Name	v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated Molecular Weight	49 kDa
GenBank Accession Number	BC000141
Gene Symbol	MYC
Gene ID (NCBI)	4609
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P01106
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.