Tested Applications
| Positive WB detected in | Calyculin A treated HEK-293 cells, Calyculin A treated NIH/3T3 cells |
| Positive IHC detected in | human prostate cancer tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | Calyculin A treated HEK-293 cells |
| Positive ChIP-qPCR detected in | Forskolin (30 μM, 1 h) HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81871-1-RR targets Phospho-CREB1 (Ser133) in WB, IHC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | cAMP responsive element binding protein 1 |
| Calculated Molecular Weight | 341 aa, 35 kDa |
| Observed Molecular Weight | 35-46 kDa |
| GenBank Accession Number | BC010636 |
| Gene Symbol | CREB1 |
| Gene ID (NCBI) | 1385 |
| RRID | AB_2935430 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P16220 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), mitogen-activated protein kinases (MAPK), and other kinases. The activity of CREB1 in neurons has been correlated with various intracellular processes, including proliferation, differentiation, survival, long-term synaptic potentiation, neurogenesis, and neuronal plasticity. CREB1 belongs to the CREB/activating transcription factor (ATF) family of transcription factors. The antibody also detects the phosphorylated form of the CREB-related protein ATF1. (PMID: 30214393, PMID: 33917483, PMID: 32605164)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Phospho-CREB1 (Ser133) antibody 81871-1-RR | Download protocol |
| IHC protocol for Phospho-CREB1 (Ser133) antibody 81871-1-RR | Download protocol |
| WB protocol for Phospho-CREB1 (Ser133) antibody 81871-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-CREB1 (Ser133) Recombinant monoclonal antibody (5H5), catalog number 81871-1-RR, has 8 total citations. These appear in journals including EMBO Molecular Medicine, Phytomedicine, Cell Oncology, PLoS Pathogens, FASEB Journal, BMC Neuroscience, Journal of Ethnopharmacology, and Cellular and Molecular Biology Letters. Associated research fields span oncology (leukemia, breast cancer), stem cell biology, reproductive medicine, pulmonary fibrosis, infectious disease, endocrinology (PCOS), and neuroscience (stroke-related depression).
| Species | Application | Title |
|---|---|---|
Cell Mol Biol Lett The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs. | ||
Phytomedicine Chinese medicine Sheng Jing Decoction alleviates oligoasthenospermia by modulating PI3K/Akt and MAPK signaling pathways. | ||
EMBO Mol Med Extracellular cyclophilin A promotes B-cell acute lymphoblastic leukemia progression via MC2R. | ||
J Ethnopharmacol Multi-omics reveals the effects and mechanisms of Xinxue Granules against idiopathic pulmonary fibrosis through activating the cAMP/EPAC/CREB axis. | ||
Cell Oncol (Dordr) P2RY6(+) tumor-associated macrophages as a therapeutic target: suppressing tumor growth and enhancing chemosensitivity in triple-negative breast cancer. | ||
