Tested Applications
| Positive WB detected in | TNF alpha and Calyculin A treated HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82090-2-RR targets Phospho-MLKL (Ser358) in WB, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | mixed lineage kinase domain-like |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | XM_005255834 |
| Gene Symbol | MLKL |
| Gene ID (NCBI) | 197259 |
| RRID | AB_3670515 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8NB16 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily. One common method for inducing necroptosis is by treating cells grown in culture with a mixture composed of TNF-α, a Smac-mimetic, that degrades cellular inhibitors of apoptosis (cIAPs) and the pancaspase inhibitor Z-VAD-FMK. This mixture is abbreviated as T/S/Z. It drives necrosome complex formation and RIPK3-dependent phosphorylation of Thr-357 and Ser-358 located within the C-terminal kinase-like domain of human MLKL. (PMID: 28878015)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-MLKL (Ser358) antibody 82090-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Phospho-MLKL (Ser358) Recombinant monoclonal antibody (241469D2), catalog number 82090-2-RR, has 24 citations. These appear in journals including Nature Cell Biology, Biomaterials, EMBO Reports, International Journal of Biological Sciences, Journal of Translational Medicine, Apoptosis, Free Radical Biology and Medicine, Cell Biosciences, Cancer Immunology & Immunotherapy, International Immunopharmacology, Cancer Science, and others. Research fields span necroptosis, PANoptosis, ferroptosis, pyroptosis, cancer biology, sepsis, liver disease, nephrology, and neuroinflammation.
| Species | Application | Title |
|---|---|---|
Biomaterials Radiation-triggerable bioreactors enable bioenergetic reprograming of cancer stem cell plasticity via targeted arginine metabolism disruption for augmented radio-immunotherapy | ||
Int J Biol Sci Curcumol Induces Necroptosis of Hepatic Stellate Cells by Targeting KAT8 to Suppress HK2 Lactylation and Promote HUWE1-Dependent Ubiquitination. | ||
J Transl Med USP22 suppresses trophoblast cell necroptosis in preeclampsia by stabilizing KAT2A-mediated histone acetylation at the SFRP1 promoter. | ||
Apoptosis Neutrophil CD11b is a pivotal PANoptosis marker correlated with disease activity in antineutrophil cytoplasmic antibody-associated vasculitis. | ||
Free Radic Biol Med Endogenous oxidized phospholipid POVPC triggers pulmonary epithelial ferroptosis in acute lung injury and is restrained by RCN3. |
