Tested Applications
| Positive WB detected in | IGF-1 treated MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82373-1-RR targets Phospho-p70(S6K) (Thr389)/p85(S6K) (Thr412) in WB, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ribosomal protein S6 kinase, 70kDa, polypeptide 1 |
| Observed Molecular Weight | 65-85 kDa |
| GenBank Accession Number | NM_003161 |
| Gene Symbol | p70 S6K |
| Gene ID (NCBI) | 6198 |
| RRID | AB_3086477 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P23443 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K), which is a serine/threonine kinase regulated by phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) pathway. P70(S6K) plays a crucial role in controlling cell cycle, growth and survival. The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation and metabolism and is the central regulator of translation of some components of protein synthesis system. Due to alternative translation two isoform S6K1 proteins are known to exist in mammalian cells: p85(S6K) and p70(S6K), which is identical to p85(S6K) but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1). mTOR is know to phosphorylate and thereby activate p70(S6K) at Thr389 and p85(S6K) at Thr412. (PMID: 25100792, PMID: 24970012, PMID: 21602892)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-p70(S6K) (Thr389)/p85(S6K) (Thr412) antibody 82373-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-p70(S6K) (Thr389)/p85(S6K) (Thr412) Recombinant monoclonal antibody (1G15), catalog number 82373-1-RR, has 8 total citations appearing in journals including Cell Death & Disease, Journal of Translational Medicine, International Journal of Biological Macromolecules, Free Radical Biology and Medicine, Cell Reports, International Journal of Molecular Sciences, Cell Biochemistry and Biophysics, and eLife. Research fields span oncology (melanoma, hepatocellular carcinoma, colorectal cancer, breast cancer), neuroscience, bone biology, ferroptosis, and infectious disease.
| Species | Application | Title |
|---|---|---|
Cell Death Dis LINC00622 transcriptionally promotes RRAGD to repress mTORC1-modulated autophagic cell death by associating with BTF3 in cutaneous melanoma | ||
J Transl Med Identification of the CYP19A1-GPER1 axis as a critical oncogenic driver in hepatocellular carcinoma via AKT activation. | ||
Int J Biol Macromol Autophagic damage in senescent bone marrow mesenchymal stromal cells: Impact on Piezo1 expression during osteoporosis progression | ||
Free Radic Biol Med Bufotenidine attenuates astrocyte ferroptosis in depressive-like behaviors through targeting GluA1 to reduce neuronal lipid synthesis. | ||
Int J Mol Sci SCAMP3-Driven Regulation of ERK1/2 and Autophagy Phosphoproteomics Signatures in Triple-Negative Breast Cancer. |
Reviews
What customers say
One review was submitted with a 1-star rating. The customer tested this phospho-S6K antibody by Western Blot at 1:2000 dilution on HeLa, HEK293, and Jurkat cell lines as well as primary T-cells. While they confirmed the corresponding total S6K antibody performed well, they reported that the phospho-S6K antibody produced an unexpected band around 90 kDa, which does not correspond to the expected S6K molecular weight of approximately 85 kDa, suggesting potential non-specific reactivity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Elke (Verified Customer) (04-10-2026) | While the total S6K antibody (14485-1-AP) works nicely, the phopsho-S6K antibody reacts with a band around 90 kDa that seems not to be S6K (around 85 kDa) when put on top to each other (see enclosed ppt).
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