Tested Applications
| Positive WB detected in | HeLa cells, rat heart tissue, A549 cells, NIH/3T3 cells, C6 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82773-1-RR targets OGDH in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag7368 Product name: Recombinant human OGDH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 674-1023 aa of BC004964 Sequence: SGQDVERGTFSHRHHVLHDQNVDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFHNTAQCIIDQFICPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPDLKEANFDINQLYDCNWVVVNCSTPGNFFHVLRRQILLPFRKPLIIFTPKSLLRHPEARSSFDEMLPGTHFQRVIPEDGPAAQNPENVKRLLFCTGKVYYDLTRERKARDMVGQVAITRIEQLSPFPFDLLLKEVQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTISRAKPVWYAGRDPAAAPATGNKKTHLTELQRLLDTAFDLDVFKNFS Predict reactive species |
| Full Name | oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) |
| Calculated Molecular Weight | 116 kDa |
| Observed Molecular Weight | 116 kDa |
| GenBank Accession Number | BC004964 |
| Gene Symbol | OGDH |
| Gene ID (NCBI) | 4967 |
| RRID | AB_3670547 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q02218 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for OGDH antibody 82773-1-RR | Download protocol |
| IHC protocol for OGDH antibody 82773-1-RR | Download protocol |
| WB protocol for OGDH antibody 82773-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The OGDH Recombinant Monoclonal Antibody (Catalog No. 82773-1-RR) has 1 total citation. It appears in the journal Advanced Science (Impact Factor 14.1), published in 2025. The cited study, "The Lactate-Primed KAT8–PCK2 Axis Exacerbates Hepatic Ferroptosis During Ischemia/Reperfusion Injury by Reprogramming OXSM-Dependent Mitochondrial Fatty Acid Synthesis," used the antibody for Western blot (WB) in human and mouse samples. The associated research field is hepatic ferroptosis and ischemia/reperjury injury, involving mitochondrial fatty acid synthesis reprogramming.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) The Lactate-Primed KAT8‒PCK2 Axis Exacerbates Hepatic Ferroptosis During Ischemia/Reperfusion Injury by Reprogramming OXSM-Dependent Mitochondrial Fatty Acid Synthesis |
