• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

VEGF Receptor 2 Recombinant antibody

VEGF Receptor 2 Recombinant Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230238D7

Cat no : 83049-4-RR

Synonyms

CD309, Fetal liver kinase 1, FLK 1, FLK1, KDR, Kinase insert domain receptor, VEGFR, VEGFR 2, VEGFR2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human placenta tissue using VEGF Receptor 2 Recombinant antibody (83049-4-RR)

    WB analysis of human placenta using 83049-4-RR

    human placenta tissue was subjected to SDS PAGE followed by western blot with 83049-4-RR (KDR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using VEGF Receptor 2 Recombinant antibody (83049-4-RR)

    IHC staining of human placenta using 83049-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (KDR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using VEGF Receptor 2 Recombinant antibody (83049-4-RR)

    IHC staining of human placenta using 83049-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (KDR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HUVEC cells using VEGF Receptor 2 Recombinant antibody (83049-4-RR)

    FC experiment of HUVEC using 83049-4-RR

    1x10^6 HUVEC cells were surface stained with 0.25 ug VEGF Receptor 2 Recombinant antibody (83049-4-RR, Clone:230238D7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83049-4-RR targets VEGF Receptor 2 in WB, FC, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name kinase insert domain receptor (a type III receptor tyrosine kinase)
Calculated Molecular Weight152kd
Observed Molecular Weight150 kDa, 230 kDa
GenBank Accession NumberNM_002253
Gene Symbol KDR
Gene ID (NCBI) 3791
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KDR, also named as VEGFR-2, FLK1 and CD309, is a receptor for VEGF or VEGFC. KDR which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.

Protocols

Product Specific Protocols
WB protocol for VEGF Receptor 2 antibody 83049-4-RRDownload protocol
IHC protocol for VEGF Receptor 2 antibody 83049-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols