Sandwich ELISA standard curve of MP00039-3, MOUSE Adiponectin Recombinant Matched Antibody Pair - PBS only. 83070-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0262. 83070-3-PBS was HRP conjugated as the detection antibody. Range:15.6-1000pg/mL.

Serum of sixteen mice was measured. The Adiponectin concentration of detected samples was determined to be 7.30 ug/mL with a range of 4.72-13.56 ug/mL.

lungs from one mouse were chopped into 1-2 mm pieces and seeded into approximately 30 mL of media containing RPMI, 10% fetal bovine serum, 50 μM β-mercaptoethanol, and L-glutamine.The mean Adiponectin concentration was determined to be 4.4 ng/mL in lung cell culture supernatant.

Organs from 2 mice were rinsed with PBS to remove excess blood, chopped into 1-2 mm pieces, homogenized in 5-10 mL of PBS in a tissue homogenizer, and stored at ≤ -80℃ 5 min. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g to remove particulate. The mean Adiponectin concentration was determined to be 130.21 ng/mL in spleen homogenates, 745.9 ng/mL in fat tissue homogenates.