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  • Western Blot (WB) analysis of various lysates using CEACAM8 Recombinant antibody (83105-6-RR)

    WB analysis using 83105-6-RR

    human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 83105-6-RR (CEACAM8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CEACAM8 Recombinant antibody (83105-6-RR)

    WB analysis using 83105-6-RR

    human saliva were subjected to SDS PAGE followed by western blot with 83105-6-RR (CEACAM8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83105-6-RR

    Affinity and Kinetic Characterization of 83105-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83105-6-RR against Human CEACAM8 were performed. The affinity constant is 13.9 nM.

CEACAM8 Recombinant antibody

CEACAM8 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83105-6-RR
Clone No.242954B2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CD66b, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, CD 66b, CD 67

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inhuman saliva, human peripheral blood leukocyte

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83105-6-RR targets CEACAM8 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CEACAM8 fusion protein Eg32133 Predict reactive species
Full Name carcinoembryonic antigen-related cell adhesion molecule 8
Calculated Molecular Weight 38 kDa
Observed Molecular Weight95-100kDa
GenBank Accession NumberBC026263
Gene Symbol CEACAM8
Gene ID (NCBI) 1088
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP31997
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8), also known as CD66b, CGM6 or NCA-95, is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAM8 is a glycosylphosphatidylinositol (GPI)-linked glycoprotein expressed on granulocytes (PMID: 8104998; 18056392). It is involved in regulating adhesion and activation of eosinophils (PMID: 18056392).

Protocols

Product Specific Protocols
WB protocol for CEACAM8 antibody 83105-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols