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INO80 Recombinant monoclonal antibody (83117-4-RR)

INO80 Recombinant monoclonal antibody

INO80 Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Cat No. 83117-4-RR

Clone No. 230440E9

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

230440E9, Chromatin-remodeling ATPase INO80, DNA helicase-related INO80 complex homolog 1, DNA helicase-related protein INO80, EC:3.6.4.-

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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Filter: AllIHCIF/ICCFC (Intra)

IHC staining of mouse pancreas using 83117-4-RR
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83117-4-RR (INO80 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse tongue using 83117-4-RR
Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 83117-4-RR (INO80 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 83117-4-RR
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 83117-4-RR (INO80 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse thymus using 83117-4-RR
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 83117-4-RR (INO80 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse tongue using 83117-4-RR
Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 83117-4-RR (INO80 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat tongue using 83117-4-RR
Immunohistochemical analysis of paraffin-embedded rat tongue tissue slide using 83117-4-RR (INO80 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 83117-4-RR
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using INO80 antibody (83117-4-RR, Clone: 230440E9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 83117-4-RR
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using INO80 antibody (83117-4-RR, Clone: 230440E9 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HeLa using 83117-4-RR
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ino80 Recombinant Antibody (83117-4-RR, Clone:230440E9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for INO80 in ?

Overview

Tested Applications
Product Information
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MSDS SDS

Tested Applications

Positive IHC detected in	mouse pancreas tissue, human pancreas cancer tissue, mouse thymus tissue, mouse tongue tissue, rat tongue tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83117-4-RR targets INO80 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	INO80 homolog (S. cerevisiae)
Calculated Molecular Weight	1556 aa, 177 kDa
GenBank Accession Number	BC146785
Gene Symbol	INO80
Gene ID (NCBI)	54617
RRID	AB_3670830
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9ULG1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.