SOX8 Recombinant monoclonal antibody

SOX8 Uni-rAb^® Recombinant Antibody for IF/ICC, IF-P, ELISA

Cat No. 83238-3-RR

Clone No. 240120H6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IF-P, ELISA

Synonyms

240120H6, Transcription factor SOX 8, Transcription factor SOX-8

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllIF-PIF/ICCAffinity

IF Staining of mouse brain using 83238-3-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SOX8 antibody (83238-3-RR, Clone: 240120H6 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 83238-3-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SOX8 antibody (83238-3-RR, Clone: 240120H6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 83238-3-RR
Biolayer interferometry (BLl) kinetic assays of 83238-3-RR against Human SOX8 were performed. The affinity constant is 1.98 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for SOX8 in ?

Positive IF-P detected in	mouse brain tissue
Positive IF/ICC detected in	HeLa cells

Application	Dilution
Immunofluorescence (IF)-P	IF-P : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

83238-3-RR targets SOX8 in IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag34395 Product name: Recombinant human SOX8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 298-446 aa of BC031797 Sequence: EPGQAYGGAYFHAGASPVWAHKSAPSASASPTETGPPRPHIKTEQPSPGHYGDQPRGSPDYGSCSGQSSATPAAPAGPFAGSQGDYGDLQASSYYGAYPGYAPGLYQYPCFHSPRRPYASPLLNGLALPPAHSPTSHWDQPVYTTLTRP Predict reactive species
Full Name	SRY (sex determining region Y)-box 8
Calculated Molecular Weight	446 aa, 47 kDa
GenBank Accession Number	BC031797
Gene Symbol	SOX8
Gene ID (NCBI)	30812
RRID	AB_3670914
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P57073
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.