Tested Applications
| Positive IHC detected in | mouse cerebellum tissue, mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:125-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83323-6-RR targets NRG1, isoform SMDF in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag0803 Product name: Recombinant human NRG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-225 aa of BC007675 Sequence: MEIYSPDMSEVAAERSSSPSTQLSADPSLDGLPAAEDMPEPQTEDGRTPGLVGLAVPCCACLEAERLRGCLNSEKICIVPILACLVSLCLCIAGLKWVFVDKIFEYDSPTHLDPGGLGQDPIISLDATAASALWVSSEAYTSPVSRAQSESEVQVTVQGDKAVVSFEPSAAPTPKNRIFAFSFLPSTAPSFPSPTRNPEVRTPKSATQPQTTETNLQTAPKLSTS Predict reactive species |
| Full Name | neuregulin 1 |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC007675 |
| Gene Symbol | NRG1 |
| Gene ID (NCBI) | 3084 |
| RRID | AB_3670989 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q02297 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform SMDF.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NRG1, isoform SMDF antibody 83323-6-RR | Download protocol |
| IHC protocol for NRG1, isoform SMDF antibody 83323-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NRG1, isoform SMDF Recombinant monoclonal antibody (240184G2), catalog number 83323-6-RR, has 1 total citation. It appears in the International Journal of Oncology (Int J Oncol), where it was used for IHC in a study on hypoxia-induced exosomal CAMTA1 promoting radio-resistance in MDA-MB-231 breast cancer cells by regulating NRG1 to mediate M2 macrophage polarization. The research field encompasses cancer biology, specifically breast cancer, hypoxia, exosome-mediated signaling, radio-resistance, and tumor-associated macrophage polarization.
| Species | Application | Title |
|---|---|---|
Int J Oncol Hypoxia‑induced exosomal CAMTA1 promotes radio‑resistance in MDA‑MB‑231 cells by regulating NRG1 to mediate M2 macrophage polarization. |
