Tested Applications
| Positive WB detected in | L02 cells, mouse liver tissue, HepG2 cells, HeLa cells, HT-1080 cells, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83329-2-RR targets Catalase in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag15383 Product name: Recombinant human Catalase protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 228-527 aa of BC112219 Sequence: EAVYCKFHYKTDQGIKNLSVEDAARLSQEDPDYGIRDLFNAIATGKYPSWTFYIQVMTFNQAETFPFNPFDLTKVWPHKDYPLIPVGKLVLNRNPVNYFAEVEQIAFDPSNMPPGIEASPDKMLQGRLFAYPDTHRHRLGPNYLHIPVNCPYRARVANYQRDGPMCMQDNQGGAPNYYPNSFGAPEQQPSALEHSIQYSGEVRRFNTANDDNVTQVRAFYVNVLNEEQRKRLCENIAGHLKDAQIFIQKKAVKNFTEVHPDYGSHIQALLDKYNAEKPKNAIHTFVQSGSHLAAREKANL Predict reactive species |
| Full Name | catalase |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC112219 |
| Gene Symbol | Catalase |
| Gene ID (NCBI) | 847 |
| RRID | AB_3743476 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04040 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes the growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells, and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which is also known as acatalasemia.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Catalase antibody 83329-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
