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  • Sandwich ELISA standard curve of MP00533-3

    Sandwich ELISA standard curve of MP00533-3

    Sandwich ELISA standard curve of MP00533-3, mouse IL-2 Recombinant Matched Antibody Pair - PBS only. 98055-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0762. 83547-1-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 pg/mL.

  • Sample test of MP00533-3

    Sample test of MP00533-3

    EL-4 mouse lymphoblast cells (1 x 10^6  cells/mL) were cultured for 3 days in DMEM supplemented with 10% fetal bovine serum and stimulated with 10 μg/mL PHA and 10 ng/mL PMA. The mean mouse IL-2 concentration was determined to be 16.1 pg/mL in unstimulated EL-4, 309.9 ng/mL in PHA and PMA stimulated EL-4 supernatant.

  • Sample test of MP00533-3

    Sample test of MP00533-3

    Serum of sixteen mice was measured. were measured. The mouse IL-2 concentration was determined to be 2.27 pg/mL with a range of 1.1-79.9 pg/mL.

  • Affinity and Kinetic Characterization of 83547-1-PBS

    Affinity and Kinetic Characterization of 83547-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 83547-1-PBS against Mouse IL-2 were performed. The affinity constant is 3.03 nM.

Mouse IL-2 Recombinant antibody, PBS Only (Detector)

IL-2 antibodies are employed to detect, quantify, and neutralize interleukin-2, a cytokine central to T cell proliferation and immune regulation, in immunology research and clinical studies. Proteintech has an extensive validated IL-2 antibodies for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test.
Cat No. 83547-1-PBS
Clone No.240358A4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Il2, Il 2, interleukin 2, Interleukin-2, T-cell growth factor

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

83547-1-PBS targets IL-2 as part of a matched antibody pair:

MP00533-3: 98055-1-PBS capture and 83547-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IL-2 fusion protein Eg0762 Predict reactive species
Full Name interleukin 2
Calculated Molecular Weight 19 kDa
GenBank Accession Number NM_008366
Gene Symbol IL-2
Gene ID (NCBI) 16183
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04351
Storage Buffer PBS only, pH 7.3.
Storage ConditionsStore at -80°C.