Sandwich ELISA standard curve of MP00791-1, Human SMAD2 Recombinant Matched Antibody Pair - PBS only. 83841-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3237. 83841-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

Serum of sixteen individual healthy human donors was measured. The human SMAD2 concentration of detected samples was determined to be 2.17 ng/mL with a range of 0.34 - 8.22 ng/mL.

HEK-293T, HeLa, HepG2 and MCF-7 cell lysates were measured. The human SMAD2 concentration of detected samples was determined to be 43.85 ng/mL in HEK-293T cell lysate (based on a 5.5 mg/mL extract load). The human SMAD2 concentration of detected samples was determined to be 33.98 ng/mL in HeLa cell lysate (based on a 7 mg/mL extract load). The human SMAD2 concentration of detected samples was determined to be 24.50 ng/mL in HepG2 cell lysate (based on a 4.0 mg/mL extract load). The human SMAD2 concentration of detected samples was determined to be 12.02 ng/mL in MCF-7 cell lysate (based on a 3.1 mg/mL extract load).

Cytometric bead array standard curve of MP00791-1, SMAD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83841-2-PBS. Detection antibody: 83841-3-PBS. Standard: Ag3237. Range: 0.469-60 ng/mL.