SOD1 Recombinant monoclonal antibody

SOD1 Uni-rAb^® Recombinant Antibody for WB, IF-P, ELISA

Cat No. 83857-6-RR

Clone No. 240888B9

Publications (1)

Review Product

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF-P, ELISA

Synonyms

240888B9, ALS1, EC:1.15.1.1, homodimer, hSod1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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WB analysis using 83857-6-RR
Various lysates were subjected to SDS PAGE followed by western blot with 83857-6-RR (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse brain using 83857-6-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SOD1 antibody (83857-6-RR, Clone: 240888B9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse brain using 83857-6-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SOD1 antibody (83857-6-RR, Clone: 240888B9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse brain using 83857-6-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SOD1 antibody (83857-6-RR, Clone: 240888B9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 83857-6-RR
Biolayer interferometry (BLl) kinetic assays of 83857-6-RR against Human SOD1 were performed. The affinity constant is 13.0 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, K-562 cells
Positive IF-P detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83857-6-RR targets SOD1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	bovine
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg1043 Product name: Recombinant Human SOD1 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: N-6His Domain: 2-154 aa of NM_000454.5 Sequence: ATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ Predict reactive species
Full Name	superoxide dismutase 1, soluble
Calculated Molecular Weight	16kDa
Observed Molecular Weight	16-20 kDa
GenBank Accession Number	NM_000454.5
Gene Symbol	SOD1
Gene ID (NCBI)	6647
RRID	AB_3743503
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P00441
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.

Protocols

Product Specific Protocols
IF protocol for SOD1 antibody 83857-6-RR	Download protocol
WB protocol for SOD1 antibody 83857-6-RR	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The SOD1 Recombinant monoclonal antibody (240888B9), catalog number 83857-6-RR, has a total of 1 citation. The cited publication appeared in Antioxidants (Basel) and investigated the development of an AAPH-induced oxidative stress model in bovine mammary epithelial cells, exploring its molecular mechanisms. The research field is oxidative stress biology, with the antibody applied in Western blot and immunofluorescence.

Species	Application	Title
bovine	WB, IF	Antioxidants (Basel) Development of an AAPH-Induced Oxidative Stress Model in Bovine Mammary Epithelial Cells and Investigation of Its Molecular Mechanisms. Authors - Yuanyuan Zhang View Article