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  • Immunohistochemistry (IHC) staining of mouse testis tissue using TICAM2 Recombinant antibody (84064-6-RR)

    IHC staining of mouse testis using 84064-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84064-6-RR (TICAM2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using TICAM2 Recombinant antibody (84064-6-RR)

    IF Staining of PC-3 using 84064-6-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using TICAM2 antibody (84064-6-RR, Clone: 241186C12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TICAM2 Recombinant antibody

TICAM2 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84064-6-RR
Clone No.241186C12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

241186C12, MyD88-4, Putative NF-kappa-B-activating protein 502, TICAM 2, TICAM-2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84064-6-RR targets TICAM2 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name toll-like receptor adaptor molecule 2
Calculated Molecular Weight 27 kDa
GenBank Accession NumberNM_021649
Gene Symbol TICAM2
Gene ID (NCBI) 353376
RRIDAB_3671634
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ86XR7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TICAM2, also named TIRAP3, TIRP, and TRAM, is involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1. TICAM2 is involved in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS.

Protocols

Product Specific Protocols
IHC protocol for TICAM2 antibody 84064-6-RRDownload protocol
IF protocol for TICAM2 antibody 84064-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols