Tested Applications
| Positive IF-P detected in | human skeletal muscle tissue |
| Positive IF/ICC detected in | HSkMC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84099-1-RR targets MYH2-specific in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | myosin, heavy chain 2, skeletal muscle, adult |
| Calculated Molecular Weight | 223 kDa |
| GenBank Accession Number | NM_017534 |
| Gene Symbol | MYH2 |
| Gene ID (NCBI) | 4620 |
| RRID | AB_3671664 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UKX2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYH2-specific antibody 84099-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYH2-specific Recombinant monoclonal antibody (Cat# 84099-1-RR) has 2 citations. One appears in Food Research International (2025), studying actin-myosin complex dissociation during cold storage of grass carp muscle. The other is in Biochemical and Biophysical Research Communications (2026), investigating tamoxifen-promoted myogenic differentiation and skeletal muscle regeneration. The associated research fields span food science/aquaculture and muscle biology/regenerative medicine.
| Species | Application | Title |
|---|---|---|
Food Res Int Actin-myosin complex dissociation initiates programmed cell death during cold storage of grass carp muscle. | ||
Biochem Biophys Res Commun Tamoxifen promotes myogenic differentiation and skeletal muscle regeneration via activation of the GRP94. |
Reviews
What customers say
One reviewer used this antibody for immunofluorescence at a 1:100 dilution on myogenic progenitors and nascent myotubes, rating it 4 out of 5. They reported inconclusive results, attributing this to the nascent myotubes in their study likely not expressing the MYH2 isoform, rather than a problem with the antibody itself.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Pavithra (Verified Customer) (08-21-2026) | Inconclusive results because the myotubes in the study were nascent and probably did not express the MYH2 isoform.
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