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  • Sandwich ELISA standard curve of MP01133-1

    Sandwich ELISA standard curve of MP01133-1

    Sandwich ELISA standard curve of MP01133-1, Human CD8b Recombinant Matched Antibody Pair - PBS only. 84240-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02351. 84240-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP01133-1

    Sample test of MP01133-1

    Serum of six individual healthy human donors was measured. The CD8b concentration of detected samples was determined to be 204 pg/mL with a range of 73-480 pg/mL.

  • Sample test of MP01133-1

    Sample test of MP01133-1

    Human peripheral blood mononuclear cells (PBMCs), with HeLa and HEK-293 as negative controls, were cultured for 5 days. The mean concentration of CD8b was measured 99.4 pg/mL in the PBMC supernatant, undetectable in HeLa and HEK-293 supernatants.

  • Cytometric bead array standard curve of MP01133-1

    Cytometric bead array standard curve of MP01133-1

    Cytometric bead array standard curve of MP01133-1, CD8B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84240-3-PBS. Detection antibody: 84240-1-PBS. Standard: RP02351. Range: 0.078-10 ng/mL.

  • Affinity and Kinetic Characterization of 84240-1-PBS

    Affinity and Kinetic Characterization of 84240-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 84240-1-PBS against Human CD8B was performed. The affinity constant is 0.693 nM.

CD8B Recombinant monoclonal antibody, PBS Only (Detector)

CD8B Uni-rAb® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84240-1-PBS
Clone No.241326C9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD8, CD8b molecule, CD8B1, Leu2, Ly3

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

84240-1-PBS targets CD8B as part of a matched antibody pair:

MP01133-1: 84240-3-PBS capture and 84240-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Recombinant Protein

Predict reactive species
Full Name CD8b molecule
Calculated Molecular Weight 243 aa, 27 kDa
GenBank Accession NumberBC100913
Gene Symbol CD8B
Gene ID (NCBI) 926
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP10966
Storage Buffer PBS only, pH 7.3.
Storage ConditionsStore at -80°C.
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