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  • Western Blot (WB) analysis of Jurkat cells using MTMR4 Recombinant antibody (84328-1-RR)

    WB analysis of Jurkat using 84328-1-RR

    WB result of MTMR4 antibody (84328-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTMR4 transfected Jurkat cells.

  • Western Blot (WB) analysis of various lysates using MTMR4 Recombinant antibody (84328-1-RR)

    WB analysis using 84328-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84328-1-RR (MTMR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of Human Hepatocellular Cancer using MTMR4 Recombinant antibody (84328-1-RR)

    IHC staining of Human Hepatocellular Cancer using 84328-1-RR

    Immunohistochemical analysis of paraffin-embedded Human Hepatocellular Cancer slide using 84328-1-RR (MTMR4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of Human Hepatocellular Cancer using MTMR4 Recombinant antibody (84328-1-RR)

    IHC staining of Human Hepatocellular Cancer using 84328-1-RR

    Immunohistochemical analysis of paraffin-embedded Human Hepatocellular Cancer slide using 84328-1-RR (MTMR4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MTMR4 Recombinant antibody (84328-1-RR)

    IF Staining of HepG2 using 84328-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MTMR4 antibody (84328-1-RR, Clone: 241304E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84328-1-RR

    Affinity and Kinetic Characterization of 84328-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84328-1-RR against Human MTMR4 were performed. The affinity constant is 2.06 nM.

  • Featured Product
  • KD/KO Validated

MTMR4 Recombinant monoclonal antibody

MTMR4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84328-1-RR
Clone No.241304E11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA0647, FYVE-DSP2, FYVE DSP2, FYVE domain-containing dual specificity protein phosphatase 2, EC:3.1.3.48

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inMCF-7 cells, Jurkat cells, HepG2 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84328-1-RR targets MTMR4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4310

Product name: Recombinant human MTMR4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 845-1195 aa of BC035609

Sequence: SHQEQLSSVPDLTHGEEDIGKRGNNRNGQLLENPRFGKMPLELVRKPISQSQISEFSFLGSNWDSFQGMVTSFPSGEATPRRLLSYGCCSKRPNSKQMRATGPCFGGQWAQREGVKSPVCSSHSNGHCTGPGGKNQMWLSSHPKQVSSTKPVPLNCPSPVPPLYLDDDGLPFPTDVIQHRLRQIEAGYKQEVEQLRRQVRELQMRLDIRHCCAPPAEPPMDYEDDFTCLKESDGSDTEDFGSDHSEDCLSEASWEPVDKKETEVTRWVPDHMASHCYNCDCEFWLAKRRHHCRNCGNVFCAGCCHLKLPIPDQQLYDPVLVCNSCYEHIQVSRARELMSQQLKKPIATASS

Predict reactive species
Full Name myotubularin related protein 4
Calculated Molecular Weight 1195 aa, 133 kDa
Observed Molecular Weight 160-170 kDa
GenBank Accession NumberBC035609
Gene Symbol MTMR4
Gene ID (NCBI) 9110
RRIDAB_3671869
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9NYA4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myotubularin-related protein-4 (MTMR4), also named FYVE-DSP2, is a member of the myotubularin family of phosphoinositide phosphatases that selectively degrade the 3-position phosphate from the inositol ring of PtdIns(3)P and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PMID: 31543504). MTMR4 knockdown markedly suppressed the motility, fusion, and fission of PI(3)P- enriched structures, resulting in decreases in late endosomes, autophagosomes, and lysosomes, and enlargement of PI(3)P- enriched early and late endosomes(PMID: 29962048). MTMR4 is an intracellular protein with a calculated molecular mass of 133 kDa, and detected molecular weight in the papers is about 160-170 kDa, which may be due to post-translational modification (PMID: 19125695, PMID: 20736309).

Protocols

Product Specific Protocols
IF protocol for MTMR4 antibody 84328-1-RRDownload protocol
WB protocol for MTMR4 antibody 84328-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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