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  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PERM1 Recombinant antibody (84425-5-RR)

    IHC staining of mouse skeletal muscle using 84425-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84425-5-RR (PERM1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PERM1 Recombinant antibody (84425-5-RR)

    IHC staining of mouse skeletal muscle using 84425-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84425-5-RR (PERM1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of H9C2 cells using PERM1 Recombinant antibody (84425-5-RR)

    IF Staining of H9C2 using 84425-5-RR

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using PERM1 antibody (84425-5-RR, Clone: 241613B6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84425-5-RR

    Affinity and Kinetic Characterization of 84425-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84425-5-RR against Human PERM1 were performed. The affinity constant is below 1 pM.

PERM1 Recombinant antibody

PERM1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84425-5-RR
Clone No.241613B6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IHC, IF/ICC, ELISA

Synonyms

C1orf170, PERM, 241613B6

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inH9C2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84425-5-RR targets PERM1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PERM1 fusion protein Ag23833 Predict reactive species
Full Name chromosome 1 open reading frame 170
GenBank Accession NumberBC006300
Gene Symbol C1orf170
Gene ID (NCBI) 84808
RRIDAB_3671952
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ5SV97
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PERM1 (PGC-1/ERR-induced regulator in muscle 1) is a muscle-specific protein induced by PGC-1α and ERRα. PERM1 protein expression is mainly restricted to heart and skeletal muscle, indicating PERM1 functions as a tissue-specific regulator of mitochondrial biogenesis in response to exercise (PMID: 33549681, 36028747). Ablation of Perm1 in mice results in reduced protein expression of lipin-1 accompanied by accumulation of specific phospholipid species (PMID: 33549681). There are two major isoforms of Perm1 protein, the short isoform is about 85 kDa, and the long isoform is 105 kDa.

Protocols

Product Specific Protocols
IHC protocol for PERM1 antibody 84425-5-RRDownload protocol
IF protocol for PERM1 antibody 84425-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols