Tested Applications
| Positive WB detected in | HEK-293 cells, SKOV-3 cells, PC-3 cells, HeLa cells, K-562 cells, DU 145 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84493-6-RR targets TTLL12 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag13804 Product name: Recombinant human TTLL12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 293-644 aa of BC001070 Sequence: DINPVVHPHGHIFKVYTDVQQVASSLTHPRFTLTQSEADADILFNFSHFKDYRKLSQERPGVLLNQFPCENLLTVKDCLASIARRAGGPEGPPWLPRTFNLRTELPQFVSYFQQRERWGEDNHWICKPWNLARSLDTHVTKSLHSIIRHRESTPKVVSKYIESPVLFLREDVGKVKFDIRYIVLLRSVRPLRLFVYDVFWLRFSNRAFALNDLDDYEKHFTVMNYDPDVVLKQVHCEEFIPEFEKQYPEFPWTDVQAEIFRAFTELFQVACAKPPPLGLCDYPSSRAMYAVDLMLKWDNGPDGRRVMQPQILEVNFNPDCERACRYHPTFFNDVFSTLFLDQPGGCHVTCLV Predict reactive species |
| Full Name | tubulin tyrosine ligase-like family, member 12 |
| Calculated Molecular Weight | 644 aa, 74 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC001070 |
| Gene Symbol | TTLL12 |
| Gene ID (NCBI) | 23170 |
| RRID | AB_3672006 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14166 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TTLL12 is a member of the tubulin tyrosine ligase (TTL) family that is highly conserved in phylogeny. TTLL12 contains 644 amino acids and has a SET-like domain and a TTL-like domain. TTLL12 is involved in the regulation of tubulin and histone modification and is a negative regulator of RNA virus-induced type I interferon. During the cell cycle, Both trimethylation of H4K20 and tubulin detyrosination levels were altered by TTLL2 expression (PMID: 23251473).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TTLL12 antibody 84493-6-RR | Download protocol |
| WB protocol for TTLL12 antibody 84493-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody 84493-6-RR (anti-TTLL12, recombinant rabbit) has 1 citation to date. It appears in Cell Death & Disease (IF 12.2), a study on TTLL12's role in stabilizing eEF1A1 to promote hepatocarcinogenesis. The research field encompasses cell death and liver cancer biology, with the antibody validated for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Cell Death Dis TTLL12 counteracts BPOZ-2 to stabilize eEF1A1 and promote hepatocarcinogenesis.
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