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IL-15 Recombinant monoclonal antibody (84512-4-RR)

IL-15 Recombinant monoclonal antibody

IL-15 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 84512-4-RR

Clone No.252837E4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

IL15, IL 15, Interleukin-15

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

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Filter: AllWBIHC

WB analysis of Recombinant protein using 84512-4-RR
Human IL-15 recombinant protein were subjected to SDS PAGE followed by western blot with 84512-4-RR (IL-15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 84512-4-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84512-4-RR (IL-15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with .
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 84512-4-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84512-4-RR (IL-15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with .
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Recombinant protein
Positive IHC detected in	human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84512-4-RR targets IL-15 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Product name: HumanKine® recombinant human IL-15 protein Source: -derived, Tag: Domain: of Sequence: Predict reactive species
Full Name	interleukin 15
Calculated Molecular Weight	18 kDa
GenBank Accession Number	BC018149
Gene Symbol	IL15
Gene ID (NCBI)	3600
RRID	AB_3743576
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P40933
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-15 (IL-15) is a cytokine that plays a significant role in the immune system, particularly in the development, proliferation, and activation of immune cells such as natural killer (NK) cells, T cells, and B cells. It is a member of the immunoregulatory cytokines family and is predominantly expressed by myeloid cells, among other cell types. IL-15 serves multiple functions, including dictating T cell response, regulating tissue repair and B cell homing, modulating inflammation, and activating NK cells.