Tested Applications
| Positive WB detected in | Jurkat cells, MCF-7 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84660-4-RR targets C3orf37 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag36362 Product name: Recombinant human C3orf37 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 100-300 aa of BC009993 Sequence: DTVMEKRSFKVPLGKGRRCVVLADGFYEWQRCQGTNQRQPYFIYFPQIKTEKSGSIGAADSPENWEKVWDNWRLLTMAGIFDCWEPPEGGDVLYSYTIITVDSCKGLSDIHHRMPAILDGEEAVSKWLDFGEVSTQEALKLIHPTENITFHAVSSVVNNSRNNTPECLAPVDLVVKKELRASGSSQRMLQWLATKSPKKED Predict reactive species |
| Full Name | chromosome 3 open reading frame 37 |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC009993 |
| Gene Symbol | C3orf37 |
| Gene ID (NCBI) | 56941 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q96FZ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HMCES, also known as C3orf37, is a protein that is associated with the protection of abasic sites. The molecular mass of HMCES is 40 kDa and it recognizes AP sites in ssDNA at replication forks and chemically modifies the lesion to generate a DNA-protein crosslink (PMID: 30554877).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for C3orf37 antibody 84660-4-RR | Download protocol |
| WB protocol for C3orf37 antibody 84660-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
