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Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, HEK-293 cells, U-87 MG cells, NIH/3T3 cells, mouse liver tissue, mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
| Positive Dot Blot detected in | peptide |
| Positive ChIP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1250-1:5000 |
| DOT BLOT | DOT BLOT : 1:10-1:100 |
| Chromatin immunoprecipitation (ChIP) | CHIP : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84740-1-RR targets Mono-Methyl-Histone H3 (Lys4) in WB, IF/ICC, ChIP, Dot Blot, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | histone cluster 1, H3a |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC066245 |
| Gene Symbol | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| RRID | AB_3672125 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P68431 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Mono-Methyl-Histone H3 (Lys4) antibody 84740-1-RR | Download protocol |
| IF protocol for Mono-Methyl-Histone H3 (Lys4) antibody 84740-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
