Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293 cells, Raji cells, Daudi cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84901-1-RR targets UVRAG in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag30648 Product name: Recombinant human UVRAG protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 198-311 aa of BC064837 Sequence: RLHRAQCAIKQTQVTVQKIGKEIEEKLRLTSTSNELKKKSECLQLKILVLQNELERQKKALGREVALLHKQQIALQDKGSAFSAEHLKLQLQKESLNELRKECTAKRELFLKTN Predict reactive species |
| Full Name | UV radiation resistance associated gene |
| Calculated Molecular Weight | 699 aa, 78 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC064837 |
| Gene Symbol | UVRAG |
| Gene ID (NCBI) | 7405 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9P2Y5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
UVRAG is a versatile protein that is involved in the regulation of different cellular pathways implicated in membrane trafficking, and is also involved in the regulation of the COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex; Its function is dependent on its binding to phosphatidylinositol 3-phosphate (PtdIns3P) (PMID:16799551; 18552835; 20643123; 24056303; 28306502).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UVRAG antibody 84901-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
