Tested Applications
| Positive WB detected in | LNCaP cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84950-3-RR targets LARP7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag10850 Product name: Recombinant human LARP7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 83-331 aa of BC006981 Sequence: LTTDGKLIARALRSSAVVELDLEGTRIRRKKPLGERPKDEDERTVYVELLPKNVNHSWIERVFGKCGNVVYISIPHYKSTGDPKGFAFVEFETKEQAAKAIEFLNNPPEEAPRKPGIFPKTVKNKPIPALRVVEEKKKKKKKKGRMKKEDNIQAKEENMDTSNTSISKMKRSRPTSEGSDIESTEPQKQCSKKKKKRDRVEASSLPEVRTGKRKRSSSEDAESLAPRSKVKKIIQKDIIKEASEASKE Predict reactive species |
| Full Name | La ribonucleoprotein domain family, member 7 |
| Calculated Molecular Weight | 582 aa, 67 kDa |
| Observed Molecular Weight | 67 kDa |
| GenBank Accession Number | BC006981 |
| Gene Symbol | LARP7 |
| Gene ID (NCBI) | 51574 |
| RRID | AB_3743876 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q4G0J3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LARP7 belongs to the LARP RNA-binding protein family, which includes the lupus LA antigen gene, and modulates the metabolism and function of a variety of RNA species [PMID:20138158]. LARP7 binds to the 7SK RNP complex, and sequesters the positive transcription elongation factor b (P-TEFb) in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation.[PMID:19416841].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LARP7 antibody 84950-3-RR | Download protocol |
| IHC protocol for LARP7 antibody 84950-3-RR | Download protocol |
| WB protocol for LARP7 antibody 84950-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-LARP7 recombinant antibody (Cat# 84950-3-RR) has 1 total citation. It appears in J Thorac Dis (IF 2.3), published in 2026 by Jinfeng He et al. The study investigates mechanisms of LARP7 in regulating non-small cell lung cancer progression, utilizing WB and IHC applications in human and mouse samples. The research field is oncology, specifically lung cancer biology.
| Species | Application | Title |
|---|---|---|
J Thorac Dis Mechanisms of LARP7 in regulating the progression of non-small cell lung cancer. |
