Tested Applications
| Positive WB detected in | mouse heart tissue, λ phosphatase treated mouse heart tissue |
| Positive FC (Intra) detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85002-1-RR targets Phospho-Cardiac Troponin I (Ser23/24) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | troponin I type 3 (cardiac) |
| Calculated Molecular Weight | 210 aa, 24 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC096165 |
| Gene Symbol | Cardiac Troponin I |
| Gene ID (NCBI) | 7137 |
| RRID | AB_3743927 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P19429 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cardiac Troponin I (cTnI) is produced by cardiac muscle. To date, most investigative effort in phosphorylation mediated regulation of cTnI function has focused on the actions of PKA and PKC. Evidence from studies in a variety of systems, ranging from reconstituted myofilament proteins to cultured myocytes or transgenic animals with heterologous expression of modified cTnI proteins, indicates that PKA-mediated functional effects (eg, decreased myofilament Ca2 sensitivity) are mediated principally through the phosphorylation of Ser22/23 (equivalent to Ser23/24, including the initiating Met, in the rodent sequence) within the unique N-terminal extension of cTnI. (PMID: 15514163)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Phospho-Cardiac Troponin I (Ser23/24) antibody 85002-1-RR | Download protocol |
| WB protocol for Phospho-Cardiac Troponin I (Ser23/24) antibody 85002-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Phospho-Cardiac Troponin I (Ser23/24) Recombinant monoclonal antibody (Cat# 85002-1-RR) has 1 citation. It appears in the Journal of Pharmaceutical Analysis, used in a study on high-throughput screening of EGFR/Ca²⁺ signaling modulators in cardiac hypertrophy via a tetrahedral DNA nanostructure-based hESC platform. The research fields encompass cardiac hypertrophy, calcium signaling, and high-throughput drug screening. The antibody was applied in Western blot for human and mouse samples.
| Species | Application | Title |
|---|---|---|
J Pharm Anal High-throughput screening of EGFR/Ca(2+) signaling modulators in cardiac hypertrophy using a tetrahedral DNA nanostructure-based hESC platform. |
