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Phospho-PAK1 (Ser144)/PAK2 (Ser141) Recombinant monoclonal antibody (85044-1-RR)

Phospho-PAK1 (Ser144)/PAK2 (Ser141) Recombinant monoclonal antibody

Phospho-PAK1 (Ser144)/PAK2 (Ser141) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85044-1-RR

Clone No. 242123D12

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PAK1 (Ser144), PAK1 (Ser144)/PAK2 (Ser141), PAK2 (Ser141), Phospho-PAK1, Phospho-PAK1 (Ser144)

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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WB analysis using 85044-1-RR
Non-treated RAW 264.7 cells and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 85044-1-RR (Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 85044-1-RR
Non-treated C6 cells and pervanadate treated C6 cells were subjected to SDS PAGE followed by western blot with 85044-1-RR (Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

pervanadate treated C6 cells, LPS treated RAW 264.7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85044-1-RR targets Phospho-PAK1 (Ser144)/PAK2 (Ser141) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	p21 protein (Cdc42/Rac)-activated kinase 1
Calculated Molecular Weight	553 aa, 62 kDa
Observed Molecular Weight	61 kDa
GenBank Accession Number	BC109299
Gene Symbol	PAK1
Gene ID (NCBI)	5058
RRID	AB_3743965
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q13153
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human PAK family is divided into the group I (PAK1 to PAK3) and group II (PAK4 to PAK6). Group I PAK share some domains that are not present in the group II members. In particular, the autoinhibitory domain (AID) is important for regulation of the kinase activity of the group I family members. Autophosphorylation at PAK1 Ser144, or at the equivalent sites for the other PAK, stabilizes the open conformation and sustains high kinase activity. Mutation of tyrosines 131 or 429 is associated with reduced dimerization and enhanced kinase activity. (PMID: 31748572)

Protocols

Product Specific Protocols
WB protocol for Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody 85044-1-RR	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The Phospho-PAK1 (Ser144)/PAK2 (Ser141) Recombinant monoclonal antibody (242123D12), catalog number 85044-1-RR, has 1 citation to date. The citation appears in Life Science Alliance (2025), in a study investigating RalGAP complexes, secretion, and primary cilia in pancreatic disease. The antibody was used for Western blot in mouse samples, placing its application within the research fields of cell signaling, pancreatic pathology, and cilia biology.