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Galectin 1 Recombinant monoclonal antibody, PBS Only (Detector) (85091-4-PBS)

Galectin 1 Recombinant monoclonal antibody, PBS Only (Detector)

Name: Galectin 1 Recombinant monoclonal antibody, PBS Only (Detector), 100ug
Brand: Proteintech
SKU: 85091-4-PBS-100UG

Galectin 1 Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Cat No. 85091-4-PBS

Clone No.260418H4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS Only

Conjugate: Unconjugated

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Filter: AllSandwich ELISA sample testSandwich ELISA

Sandwich ELISA sample test of MP03369-2
Human milk of six individual healthy human donors was measured. The Galectin-1 concentration of detected samples was determined to be 7.97 ng/mL with a range of 0.34-22.03 ng/mL
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sandwich ELISA sample test of MP03369-2
SW480 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for human Galectin-1, and measured 2.21 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sandwich ELISA sample test of MP03369-2
Plasma of six individual healthy human donors was measured. The Galectin-1 concentration of detected samples was determined to be 15.73 ng/mL with a range of 5.90-25.74 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sandwich ELISA standard curve of MP03369-2
Sandwich ELISA standard curve of MP03369-2, Human Galectin-1 Recombinant Matched Antibody Pair - PBS only. 85091-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg7626. 85091-4-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

85091-4-PBS targets Galectin 1 as part of a matched antibody pair:

MP03369-2: 85091-3-PBS capture and 85091-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg7626 Product name: Recombinant human Galectin 1 protein Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 2-135 aa of BC020675 Sequence: ACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD Predict reactive species
Full Name	lectin, galactoside-binding, soluble, 1
Calculated Molecular Weight	15 kDa
GenBank Accession Number	BC020675
Gene Symbol	Galectin-1
Gene ID (NCBI)	3956
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P09382
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.