• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Filter:
  • Western Blot (WB) analysis of various lysates using TORC2 Recombinant antibody (85150-4-RR)

    WB analysis using 85150-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85150-4-RR (CRTC2,TORC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using TORC2 Recombinant antibody (85150-4-RR)

    FC experiment of HeLa using 85150-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TORC2 Recombinant antibody (85150-4-RR, Clone:242808B6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 85150-4-RR

    Affinity and Kinetic Characterization of 85150-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85150-4-RR against Human TORC2 were performed. The affinity constant is 0.422 nM.

TORC2 Recombinant antibody

TORC2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 85150-4-RR
Clone No.242808B6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

CRTC2, CRTC2,TORC2, 242808B6, CREB-regulated transcription coactivator 2, TORC 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Please visit your regions distributor:

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, Raji cells, A-673 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85150-4-RR targets TORC2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TORC2 fusion protein Ag3167 Predict reactive species
Full Name CREB regulated transcription coactivator 2
Calculated Molecular Weight 693 aa, 73 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC053562
Gene Symbol TORC2
Gene ID (NCBI) 200186
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ53ET0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .

Protocols

Product Specific Protocols
WB protocol for TORC2 antibody 85150-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols