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KCNV1 Recombinant monoclonal antibody (85153-3-RR)

KCNV1 Recombinant monoclonal antibody

KCNV1 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 85153-3-RR

Clone No. 242865E3

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

242865E3, HNKA, KCNB3, KV2.3, KV8.1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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WB analysis using 85153-3-RR
Various lysates were subjected to SDS PAGE followed by western blot with 85153-3-RR (KCNV1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 85153-3-RR
Various lysates were subjected to SDS PAGE followed by western blot with 85153-3-RR (KCNV1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 85153-3-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85153-3-RR (KCNV1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat brain using 85153-3-RR
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 85153-3-RR (KCNV1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 85153-3-RR
Biolayer interferometry (BLl) kinetic assays of 85153-3-RR against Human KCNV1 were performed. The affinity constant is 0.70 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications
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MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, U-87 MG cells, rat brain tissue, fetal human brain tissue, C6 cells
Positive IHC detected in	rat brain tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85153-3-RR targets KCNV1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag3376 Product name: Recombinant human KCNV1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-210 aa of BC028739 Sequence: MPSSGRALLDSPLDSGSLTSLGSSVFCSEGEGEPLALGDCFTVNVGGSRFVLSQQALSCFPHTRLGKLAVVVASYRRPGALAAVPSPLELCDDANPVDNEYFFDRSSQAFRYVLHYYRTGRLHVMEQLCALSFLQEIQYWGIDELSIDSCCRDRYFRRKELSETLDFKKDTEDQESQHESEQDFSQGPCPTVRQKLWNILEKPGSSTAAR Predict reactive species
Full Name	potassium channel, subfamily V, member 1
Calculated Molecular Weight	500 aa, 56 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	BC028739
Gene Symbol	KCNV1
Gene ID (NCBI)	27012
RRID	AB_3744060
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q6PIU1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Potassium voltage-gated channel subfamily V member 1 (KCNV1, also known as Kv8.1) is a voltage-gated potassium channel that plays a role in the repolarization phase of the action potential (PMID: 39003683). It is involved in regulating neuronal excitability and is essential for maintaining normal electrical signaling in the nervous system (PMID: 38911266). The function of KCNV1 may also include inhibiting specific types of outwardly rectifying potassium channels (PMID: 8670833).