Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293T cells, K-562 cells, Raji cells, RAW 264.7 cells, rat testis tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85332-2-RR targets UBE4B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | UBE4B fusion protein Ag12836 Predict reactive species |
| Full Name | ubiquitination factor E4B (UFD2 homolog, yeast) |
| Calculated Molecular Weight | 1173 aa, 133 kDa |
| Observed Molecular Weight | 140-146 kDa |
| GenBank Accession Number | BC093696 |
| Gene Symbol | UBE4B |
| Gene ID (NCBI) | 10277 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95155 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
UBE4B (Ubiquitin Conjugation E4 B) is a protein-coding gene that plays a crucial role in the ubiquitination process, which is essential for protein degradation and regulation of various cellular processes. UBE4B functions as both an E3 and E4 ubiquitin ligase. It collaborates with other ubiquitin ligases to catalyze the transfer of ubiquitin molecules to target proteins, leading to their degradation or other intracellular sorting events. For example, UBE4B has been shown to ubiquitinate and degrade proteins such as Ku70, c-FLIPL, and p53. It also interacts with the E3 ubiquitin ligase ITCH to induce polyubiquitination and proteasomal degradation of target proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UBE4B antibody 85332-2-RR | Download protocol |
| IF protocol for UBE4B antibody 85332-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
