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Tested Applications
| Positive WB detected in | IMR-32 cells, SH-SY5Y cells |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85375-1-RR targets NTN1-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | netrin 1 |
| Calculated Molecular Weight | 68 kDa |
| Observed Molecular Weight | 65-68 kDa |
| GenBank Accession Number | NM_004822 |
| Gene Symbol | NTN1 |
| Gene ID (NCBI) | 9423 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95631 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NTN1 is also named as NTN1L. Netrins control the guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serves as a survival factor through its association with its receptors, which prevents the initiation of apoptosis. NTN1 is involved in colorectal tumorigenesis by regulating apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NTN1-Specific antibody 85375-1-RR | Download protocol |
| WB protocol for NTN1-Specific antibody 85375-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
