KD/KO Validated

AUH Recombinant monoclonal antibody

AUH Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 85421-1-RR

Clone No. 242854F12

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

242854F12, 3-MG-CoA hydratase, AU-binding protein/enoyl-CoA hydratase, AU-specific RNA-binding enoyl-CoA hydratase, EC:4.2.1.18

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF-PAffinity

WB analysis using 85421-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 85421-1-RR (AUH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 85421-1-RR
WB result of AUH antibody (85421-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AUH transfected HepG2 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 85421-1-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85421-1-RR (AUH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse brain using 85421-1-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using AUH antibody (85421-1-RR, Clone: 242854F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 85421-1-RR
Biolayer interferometry (BLl) kinetic assays of 85421-1-RR against Human AUH were performed. The affinity constant is 29.3 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue, fetal human brain tissue, human kidney tissue
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85421-1-RR targets AUH in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag11265 Product name: Recombinant human AUH protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-310 aa of BC020722 Sequence: MAAAVAAAPGALGSLHAGGARLVAACSAWLCPGLRLPGSLAGRRAGPAIWAQGWVPAAGGPAPKRGYSSEMKTEDELRVRHLEEENRGIVVLGINRAYGKNSLSKNLIKMLSKAVDALKSDKKVRTIIIRSEVPGIFCAANLPVPTIAAIDGLALGGGLELALACDIRVAASSAKMGLVETKLAIIPGGGGTQRLPRAIGMSLAKELIFSARVLDGKEAKAVGLISHVLEQNQEGDAAYRKALDLAREFLPQGPVAMRVAKLAINQGMEVDLVTGLAIEEACYAQTIPTKDRLEGLLAFKEKRPPRYKGE Predict reactive species
Full Name	AU RNA binding protein/enoyl-Coenzyme A hydratase
Calculated Molecular Weight	310 aa, 33 kDa
Observed Molecular Weight	32 kDa
GenBank Accession Number	BC020722
Gene Symbol	AUH
Gene ID (NCBI)	549
RRID	AB_3744204
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q13825
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for AUH antibody 85421-1-RR	Download protocol
IHC protocol for AUH antibody 85421-1-RR	Download protocol
WB protocol for AUH antibody 85421-1-RR	Download protocol