Tested Applications
| Positive WB detected in | H2O2 treated Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85679-1-RR targets Phospho-LCK (Tyr505) in applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | lymphocyte-specific protein tyrosine kinase |
| Calculated Molecular Weight | 539 aa, 56 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC013200 |
| Gene Symbol | LCK |
| Gene ID (NCBI) | 3932 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P06239 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Lck is comprised of a SH3 domain, binding prolinerich regions, a SH2 domain, binding tyrosine-phosphorylated sequences, a kinase domain, a unique domain, and the negative regulatory tail. The kinase domain of Lck contains 2 important tyrosine residues. Tyr394 and Tyr505 represent the activating and inhibitory tyrosine residue, respectively. Therefore, Lck can exist in 4 distinct states of activity: an inactive state, a primed state, an active state, and a dually phosphorylated active state . Transitions between activity states are governed by phosphorylation at the Tyr394 and Tyr505 residues. When Tyr394 is dephosphorylated while Tyr505 is phosphorylated, Lck is in an inactive state. Lck becomes primed when Tyr505 is dephosphorylated and fully active after Tyr394 phosphorylation. In addition, there is a dually phosphorylated active form of Lck when both Tyr394 and Tyr505 residues are phosphorylated. (PMID: 32794043)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-LCK (Tyr505) antibody 85679-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
