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KAP1 Recombinant monoclonal antibody, PBS Only (Detector) (85693-2-PBS)

KAP1 Recombinant monoclonal antibody, PBS Only (Detector)

Name: KAP1 Recombinant monoclonal antibody, PBS Only (Detector), 100ug
Brand: Proteintech
SKU: 85693-2-PBS-100UG

KAP1 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85693-2-PBS

Clone No.243043A7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

TRIM28, E3 SUMO-protein ligase TRIM28, EC:2.3.2.27, KAP 1, KAP-1

Formulation: PBS Only

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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Proteintech Guarantee

Filter: AllSandwich ELISASample testCytometric bead arrayAffinity

Sandwich ELISA standard curve of MP02033-2
Sandwich ELISA standard curve of MP02033-2, Human KAP1 Recombinant Matched Antibody Pair - PBS only. 85693-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7350. 85693-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sample test of MP02033-2
The mean KAP1 concentration was determined to be 262.9 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load, 240.8 ng/mL in HepG2 cell extract based on a 1.8 mg/mL extract load and 281.5 ng/mL in A549 cell extract based on a 3.1 mg/mL extract load.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array standard curve of MP02033-2
Cytometric bead array standard curve of MP02033-2, KAP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85693-1-PBS. Detection antibody: 85693-2-PBS. Standard: Ag7350. Range: 0.625-80 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 85693-2-PBS
Biolayer interferometry (BLl) kinetic assay of 85693-2-PBS against Human KAP1 was performed. The affinity constant is 0.332 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

85693-2-PBS targets KAP1 as part of a matched antibody pair:

MP02033-2: 85693-1-PBS capture and 85693-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag7350 Product name: Recombinant human KAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 484-835 aa of BC004978 Sequence: KVPRVSLERLDLDLTADSQPPVFKVFPGSTTEDYNLIVIERGAAAAATGQPGTAPAGTPGAPPLAGMAIVKEEETEAAIGAPPTATEGPETKPVLMALAEGPGAEGPRLASPSGSTSSGLEVVAPEGTSAPGGGPGTLDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPANQRKCERVLLALFCHEPCRPLHQLATDSTFSLDQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAFGDTKFSAVLVEPPPMSLPGAGLSSQELSGGPGDGP Predict reactive species
Full Name	tripartite motif-containing 28
Calculated Molecular Weight	89 kDa
GenBank Accession Number	BC004978
Gene Symbol	KAP1
Gene ID (NCBI)	10155
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q13263
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.