Tested Applications
| Positive WB detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85845-1-RR targets Nephrin in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag18994 Product name: Recombinant human NPHS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 89-374 aa of BC156935 Sequence: PARGEFHLHIEACDLSDDAEYECQVGRSEMGPELVSPRVILSILVPPKLLLLTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALEAPIKASFTVNVLFPPGPPVIEWPGLDEGHVRAGQSLELPCVARGGNPLATLQWLKNGQPVSTAWGTEHTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSASQTENKNVTLSCVSKSSRPRVLLRW Predict reactive species |
| Full Name | nephrosis 1, congenital, Finnish type (nephrin) |
| Calculated Molecular Weight | 1241 aa, 135 kDa |
| Observed Molecular Weight | 120-130 kDa |
| GenBank Accession Number | BC156935 |
| Gene Symbol | Nephrin |
| Gene ID (NCBI) | 4868 |
| RRID | AB_3744418 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O60500 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is the podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Nephrin antibody 85845-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Nephrin Recombinant monoclonal antibody (250064G12), catalog number 85845-1-RR, has a total of 3 citations. These appear in Journal of Ethnopharmacology, Journal of Cellular Communication and Signaling, and Biochemical and Biophysical Research Communications. The cited studies focus on nephrology and podocyte biology, specifically investigating diabetic nephropathy, podocyte ferroptosis in diabetic kidney disease, and podocyte injury in membranous nephropathy. All three citations used the antibody for Western blot applications in human or mouse samples.
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Senkyunolide a from danggui buxue decoction protects podocytes against diabetic nephropathy via the mir-223-3p/nlrp3 inflammasome axis. | ||
J Cell Commun Signal Urinary exosomes aggravate diabetic kidney disease by inducing podocyte ferroptosis via the miR-217/SIRT1/Nrf2 pathway. | ||
Biochem Biophys Res Commun Suchilactone ameliorates podocyte injury in membranous nephropathy by modulating the JNK/p38 MAPK pathway in vitro. |
