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Tested Applications
| Positive WB detected in | rat stomach tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85868-1-RR targets MUC5AC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | mucin 5AC, oligomeric mucus/gel-forming |
| Calculated Molecular Weight | 527 kDa |
| Observed Molecular Weight | 290 kDa |
| GenBank Accession Number | NM_017511 |
| Gene Symbol | MUC5AC |
| Gene ID (NCBI) | 4586 |
| RRID | AB_3744433 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P98088 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MUC5AC (Mucin-5AC ) is a gel-forming glycoprotein of the gastric and respiratory epithelium. It can protect mucous membranes from infection and chemical damage by binding to inhaled microorganisms and particles, which are then cleared by the mucociliary system. (PubMed:14535999,PubMed:14718370)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MUC5AC antibody 85868-1-RR | Download protocol |
| WB protocol for MUC5AC antibody 85868-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Mol Med Rep Cigarette smoke combined with Klebsiella pneumoniae induce damage to the air‑blood barrier in chronic obstructive pulmonary disease rats via the MAPK/NF‑κB/IκBα pathway . | ||
J Ethnopharmacol Mogrosides from S. grosvenorii ameliorate acute pharyngitis via the PI3K/AKT-NF-κB-NLRP3 signaling pathway and oxidative stress modulation. |
