Tested Applications
| Positive WB detected in | human plasma |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85974-3-RR targets ADAMTS13 in applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | ADAMTS13 fusion protein Eg2556 Predict reactive species |
| Full Name | ADAM metallopeptidase with thrombospondin type 1 motif, 13 |
| Calculated Molecular Weight | 154 kDa |
| Observed Molecular Weight | 180 kDa |
| GenBank Accession Number | NM_139025 |
| Gene Symbol | ADAMTS13 |
| Gene ID (NCBI) | 11093 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q76LX8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin type 1 motifs, member 13) is a zinc-dependent metalloproteinase that contains multiple domains, such as the signal peptide, propeptide, metalloproteinase domain, disintegrin-like domain, and TSP-1 domain, among others. The primary function of ADAMTS13 is to specifically cleave the ultra-large multimers of von Willebrand factor (vWF), preventing excessive platelet aggregation and the formation of microvascular thrombi. Deficiency of ADAMTS13 is closely associated with thrombotic thrombocytopenic purpura (TTP), a severe disease characterized by thrombocytopenia, microvascular thrombosis, and tissue damage. Additionally, reduced ADAMTS13 activity is also linked to diseases such as myocardial infarction and stroke.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ADAMTS13 antibody 85974-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
