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OGFR Recombinant monoclonal antibody, PBS Only (Capture) (85998-1-PBS)

Filter: AllWBIPIHCIF/ICCCytometric bead array sample testCytometric bead arrayAffinity

WB analysis using 85998-1-RR (same clone as 85998-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 85998-1-RR (OGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85998-1-PBS in a different storage buffer formulation.
IP experiment of COLO 320 using 85998-1-RR (same clone as 85998-1-PBS)
IP result of anti-OGFR (IP:85998-1-RR, 4ug; Detection:85998-1-RR 1:3000) with COLO 320 cells lysate 1920 ug. This data was developed using the same antibody clone with 85998-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 85998-1-RR (same clone as 85998-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85998-1-RR (OGFR antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85998-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 85998-1-RR (same clone as 85998-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85998-1-RR (OGFR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85998-1-PBS in a different storage buffer formulation.
IF Staining of L02 using 85998-1-RR (same clone as 85998-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed L02 cells using OGFR antibody (85998-1-RR, Clone: 250452E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85998-1-PBS in a different storage buffer formulation.
Cytometric bead array sample test of MP02789-1
The mean OGFR concentration was determined to be 27.9 ng/mL in COLO 320 cell extract based on a 1.5 mg/mL extract load, 11.8 ng/mL in A549 cell extract based on a 1.2 mg/mL extract load, 21.5 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load.
Cytometric bead array standard curve of MP02789-1
Cytometric bead array standard curve of MP02789-1, OGFR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85998-1-PBS. Detection antibody: 85998-2-PBS. Standard: Ag1671. Range: 0.469-60 ng/mL.
Affinity and Kinetic Characterization of 85998-1-RR
Biolayer interferometry (BLl) kinetic assays of 85998-1-RR against Human OGFR were performed. The affinity constant is 0.211 nM.

OGFR Recombinant monoclonal antibody, PBS Only (Capture)

Name: OGFR Recombinant monoclonal antibody, PBS Only (Capture)
Brand: Proteintech
SKU: 85998-1-PBS

OGFR Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Cat No. 85998-1-PBS

Clone No.250452E7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Synonyms

Opioid growth factor receptor, Protein 7 60, Protein 7-60, Zeta type opioid receptor, Zeta-type opioid receptor

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

85998-1-PBS targets OGFR as part of a matched antibody pair:

MP02789-1: 85998-1-PBS capture and 85998-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag1671 Product name: Recombinant human OGFR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 358-658 aa of BC014137 Sequence: LERSQGDEAGGHGEDRPEPLSPKESKKRKLELSRREQPPTEPGPQSASEVEKIALNLEGCALSQGSLRTGTQEVGGQDPGEAVQPCRQPLGARVADKVRKRRKVDEGAGDSAAVASGGAQTLALAGSPAPSGHPKAGHSENGVEEDTEGRTGPKEGTPGSPSETPGPSPAGPAGDEPAESPSETPGPRPAGPAGDEPAESPSETPGPSPAGPTRDEPAESPSETPGPRPAGPAGDEPAESPSETPGPRPAGPAGDEPAESPSETPGPSPAGPTRDEPAKAGEAAELQDAEVESSAKSGKP Predict reactive species
Full Name	opioid growth factor receptor
Calculated Molecular Weight	73 kDa
Observed Molecular Weight	110 kDa
GenBank Accession Number	BC014137
Gene Symbol	OGFR
Gene ID (NCBI)	11054
RRID	AB_3744536
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9NZT2
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.