Tested Applications
| Positive WB detected in | mouse blood, rat plasma |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86041-1-RR targets LCAT in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg2756 Product name: Recombinant Mouse LCAT protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 25-438 aa of NM_008490.2 Sequence: FWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNRLEAKLDKPDVVNWMCYRKTEDFFTIWLDFNLFLPLGVDCWIDNTRIVYNHSSGRVSNAPGVQIRVPGFGKTESVEYVDDNKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLAPHQQDEYYKKLAGLVEEMYAAYGKPVFLIGHSLGCLHVLHFLLRQPQSWKDHFIDGFISLGAPWGGSIKAMRILASGDNQGIPILSNIKLKEEQRITTTSPWMLPAPHVWPEDHVFISTPNFNYTVQDFERFFTDLHFEEGWHMFLQSRDLLERLPAPGVEVYCLYGVGRPTPHTYIYDHNFPYKDPVAALYEDGDDTVATRSTELCGQWQGRQSQPVHLLPMNETDHLNMVFSNKTLEHINAILLGAYRTPKSPAASPSPPPPE Predict reactive species |
| Full Name | lecithin cholesterol acyltransferase |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 55-65 kDa |
| GenBank Accession Number | NM_008490.2 |
| Gene Symbol | Lcat |
| Gene ID (NCBI) | 16816 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P16301 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LCAT (Lecithin-cholesterol acyltransferase) is a lipoprotein-associated enzyme responsible for the synthesis of most of the plasma cholesteryl esters (CE), and as a consequence, for the maturation of high density-lipoproteins (HDL). LCAT is a 416 amino acid protein that binds to lipoproteins or is present in lipid-free form in plasma and is secreted by the liver in humans (PMID: 34256778, 26607351). Both mice and rabbits transgenic for human LCAT have marked increases in plasma HDL cholesterol and apolipoprotein (apo) A-I levels (PMID: 9054454).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LCAT antibody 86041-1-RR | Download protocol |
| WB protocol for LCAT antibody 86041-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
