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Napsin A Recombinant monoclonal antibody (86052-4-RR)

Napsin A Recombinant monoclonal antibody

Napsin A Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, IF-P, ELISA

Cat No. 86052-4-RR

Clone No. 242433C10

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, IF-P, ELISA

Synonyms

NAPSA, Asp 4, ASP4, Aspartyl protease 4, EC:3.4.23.-

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

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Filter: AllIHCIF-PIF/ICCAffinity

IHC staining of human kidney using 86052-4-RR
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86052-4-RR (Napsin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 86052-4-RR
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86052-4-RR (Napsin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung using 86052-4-RR
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 86052-4-RR (Napsin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung using 86052-4-RR
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 86052-4-RR (Napsin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of human kidney using 86052-4-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human kidney tissue using Napsin A antibody (86052-4-RR, Clone: 242433C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of human kidney using 86052-4-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human kidney tissue using Napsin A antibody (86052-4-RR, Clone: 242433C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of A549 using 86052-4-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Napsin A antibody (86052-4-RR, Clone: 242433C10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity of 86052-4-RR
Biolayer interferometry (BLI) kinetic assays of 86052-4-RR against Human Napsin A were performed. The affinity constant is 0.149 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for Napsin A in ?

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive IHC detected in	human lung tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human kidney tissue
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86052-4-RR targets Napsin A in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag9721 Product name: Recombinant human NAPSA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-420 aa of BC017842 Sequence: MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRTLNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG Predict reactive species
Full Name	napsin A aspartic peptidase
Calculated Molecular Weight	420 aa, 45 kDa
Observed Molecular Weight	40-45 kDa
GenBank Accession Number	BC017842
Gene Symbol	Napsin A
Gene ID (NCBI)	9476
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O96009
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).